Yes, I use teflon sheet, and a combination of different lubes that mix together well. What lube depends on what part of the horn, in part, and whether for a pivot screw or rod screw.
They make a difference, but less than properly fitting tubes and properly fitted keywork overall. I've also seen goofy uses of teflon that actually create friction rather than reducing it. Any contact creates friction, obviously, so if you're using a special material to minimize it you wouldn't want to use it in a nonsensical way.
Teflon compared to traditional cork, used the same exact way, seems to reduce friction by about 50% -- this is just a casual observation.
Teflon's handy for a bunch of different little idiosyncratic things that can affect player perception and "feel." One example's when, on a modern tenor, the G feels "squeaky," like it has a click, and it's due to the cork touch sort of squeaking against the cup below it. Sometimes players feel this and it feels to them like the pearl is loose. A good player will definitely notice it, like the proverbial pea in the proverbial princess's bed. A little teflon landing pad between the cork foot and the cup and the pea is gone. Something as inconsequential as that can actually be a big distraction, or the removal of one, for some players.