Dear friends, a note is the black dot on the paper!! The TONE is what you want to tune!!! When playing in a satb saxophone quartett, all instruments tune on their tones F2. Why? Because it the the most stable tone on the instrument. If you overblow on low Bb, the first tone will be that F and not the octave. If you are practicing alone, use a tuner and check all tones. To know if the mouthpiece is sitting on the optimal spot, again overblow on low Bb and compare the overtones to the same fingered pitches. If they do not match, try putting the mouthpiece according to what you hear, too low, too high. When the overtones and the fingered pitches line up, your mouthpiece is sitting on the optimal spot. This should also be done before you buy an instrument, its a test to see if the scale is even. I am sure you all know that when you tune with a tuner, your tone must be absolutly quiet, no waves, no matter if it is unison or an interval with which you are tuning.